Preparation of smear: there are several dyes that can be used to stain the bacteria simple staining technique utilizes single basic dye such as crystal violet, methylene blue, basic fuchsin etc all bacteria take up the basic dye uniformly and appear in the same colour only the morphology of the bacteria can be appreciated upon staining. Microbial survey, smear preparation, and simple stain instructional objectives 1 define roccal = green, liquid disinfectant pathogen = an agent which causes disease. Home » microbial survey, smear preparation, and simple stain microbial survey, smear preparation, and simple stain. Since staining of bacterial cells is relatively fast, inexpensive, and simple, it is the most commonly used technique to visualize bacterial cells staining not only makes bacteria more easily seen, but it allows their morphology (eg size and shape) to be visualized more easily. Exercise 3 preparation of smears and simple staining 1 which bacterium is a rod answer: bacillus megaterium 2 of what value is a simple stain answer: the value of simple stain is to allow us the determination of cell morphology, size and arrangement of organisms with the application of only one reagent such as methylene.
Preparation of bacterial smear and staining : simple & negative staining negative staining bacteria consists of clear protoplasmic matter, differing but slightly in refractive index from the medium in which they are growing, it is difficult with the ordinary microscope except. Lab 2 – microbial survey, smear preparation, and simple stain 1 define: a) pathogen – disease causing organism b) wet mount slide- microscope slide of liquid specimen covered with piece of cover glass c) yeast – unicellular fungi that reproduces asexual by budding. View lab report - form_lab_02_-_w16 from biology 2400 at macomb community college lab 2 microbial survey, smear preparation, and simple stain 1 define: a) pathogen organism that is caused by.
Start studying lab 2: microbial survey, smear prep and simple stain learn vocabulary, terms, and more with flashcards, games, and other study tools. Smear preparation the preparation of a smear is required for many laboratory procedures, including the gram-stain the purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. Laboratory exercises in microbiology 10 th edition by john harley smear preparation and simple staining 8 gram stain 9 acid-fast staining (ziehl-neelsen and kinyoun) procedures aerobic and anaerobic endospore-forming bacteria part ten survey of selected eucaryotic microorganisms 59 fungi (ascomycota also ascomycetes) i: yeasts. The oxidase test can be used to distinguish bacteria: positive organisms and negative organisms dependent upon the color obtained in the test add one drop of hydrogen peroxide to the smear determine if the organism is catalase or catalase-negative based microbial survey, smear preparation, and simple stain differential psychology.
Simple stain procedure: procedure: 1 clean and dry microscope slides thoroughly 2 flame the surface in which the smear is to be spread 3 flame the inoculating loop 4 transfer a loop full of tap water to the flamed slide surface disperse the bacteria on the loop in the drop of water on the slide and spread the drop over an area the size. Bacterial smear preparation and simple staining essay observation of bacterial cells via microscope in this experiment, we have learned several basic molecular biology techniques - bacterial smear preparation and simple staining essay introduction here they are simple staining: only one type of dye used for simple staining and it stains all cells the same color. The preparation of a bacterial smear for staining: the simple stain, gram stain, acid fast stain, capsule stain and endospore stain using methylene blue, prepare a simple stain of bacteria 3 using the gram stain method, prepare a differential stain activities for part 2: and too little can result in a smear where bacteria will be.
The basic requirements for staining are clean grease-free slide, bacteria tobe stained, inoculating loops and bunsen burner to sterilise inoculating loops before and after smear preparation two ways to fixing the slides are heat fixation and chemical fixation. Preparation of specimens here is your first exposure to the preparation of a bacterial smear and subsequent staining of it you are making a simple stain, using only one dye. A bacterial smear on a slide is normally made every time before one stains bacteria for microscopic observation besides placing bacteria on a slide, this procedure dries and fixes the bacteria to the glass, so that the cells are less likely to be washed away during the staining procedure.
Smear preparation the preparation of a smear is required for many laboratory procedures, including the gram-stain the purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure a smear can be prepared from a solid or broth medium. And keeps the bacteria stuck to the slide so that simple or complex staining procedures can be carried out on these bacteria figure 1 preparation of the heat fixed smear after preparation of the heat fixed smear, the cells may then be stained the stains are smear the bacteria on the end of the loop into one of the drops of water on the. Once a bacterial sample is heat fixed to a microscope slide, there are many staining techniques that can be used to view the bacteria a simple stain is the use of one dye to increase contrast between the specimen and background when viewed through a microscope. 4 bacterial smears and simple stains introduction the refractive index of most bacteria is only slightly greater than that of water therefore bacteria are difficult to see in liquid suspension.
Lab exercise 5: the smear and simple staining lab exercise 5: the smear and simple staining background smears the preparation of a good smear is the backbone of all of the staining techniques performed throughout this course a good. This feature is not available right now please try again later. Bacillus subtilis stained with simple and gram stain protocol: preparation of sample for staining: heat fixing 1 label slide: use permanent marker to write name of sample on one end of the use brightfield microscope to examine cell smear begin with low magnification of bacteria from a concentrated suspension of cells to achieve.